Publications by authors named "A Tissier"
The intracellular colonization of plant roots by the beneficial fungal endophyte Serendipita indica follows a biphasic strategy, including a host cell death phase that enables successful colonization of Arabidopsis thaliana roots. How host cell death is initiated and controlled is largely unknown. Here, we show that two fungal enzymes, the ecto-5'-nucleotidase SiE5NT and the nuclease SiNucA, act synergistically in the apoplast at the onset of cell death to produce deoxyadenosine (dAdo).
View Article and Find Full Text PDFMethylerythritol cyclodiphosphate (MEcPP) is an intermediate in the biosynthesis of isoprenoids in plant plastids and in bacteria, and acts as a stress signal in plants. Here, we show that MEcPP regulates biofilm formation in Escherichia coli K-12 MG1655. Increased MEcPP levels, triggered by genetic manipulation or oxidative stress, inhibit biofilm development and production of fimbriae.
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- The development of new anti-cancer drugs is hindered by challenges in identifying their mode of action (MoA), but a combination of metabolomics and machine learning has been used to predict MoAs for new drug candidates targeting prostate cancer cells.
- In a study of 38 drugs affecting cancer metabolism, distinct metabolic patterns enabled accurate predictions of MoAs, and these predictions were validated across other cancer types such as breast cancer and Ewing's sarcoma, albeit with some loss in prediction quality.
- This approach not only enhances understanding of drug effects on cellular processes, such as mitochondrial dysfunction, but also opens up new avenues for optimizing drug combinations to improve treatment outcomes.
RNA-guided endonucleases originating from the bacterial CRISPR/Cas system are a versatile tool for targeted gene editing. To determine the functional relevance of a gene of interest, deletion of the entire open reading frame (ORF) by two independent double-strand breaks (DSBs) is particularly attractive. This strategy greatly benefits from high editing efficiency, which is strongly influenced by the Cas endonuclease version used.
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