Publications by authors named "A Timothy Leard"
Background: Sarcoid tumors are common in horses and may negatively impact the performance and value of the horse. No known treatment is reliably successful.
Hypotheses/objectives: To determine tolerability, overall response rate, time to response, and progression-free survival of horses with biopsy-confirmed or suspected sarcoids treated with ALVAC-fIL2.
The reactivity of platelets, which play a key role in the pathogenesis of atherothrombosis, is tightly regulated. The integral membrane protein tetherin/bone marrow stromal antigen-2 (BST-2) regulates membrane organization, altering both lipid and protein distribution within the plasma membrane. Because membrane microdomains have an established role in platelet receptor biology, we sought to characterize the physiological relevance of tetherin/BST-2 in those cells.
View Article and Find Full Text PDFThe neuropeptide galanin has diverse roles in the central and peripheral nervous systems, by activating the G protein-coupled receptors Gal1, Gal2 and the less studied Gal3 (GalR1-3 gene products). There is a wealth of data on expression of Gal1-3 at the mRNA level, but not at the protein level due to the lack of specificity of currently available antibodies. Here we report the generation of knock-in mice expressing Gal1 or Gal2 receptor fluorescently tagged at the C-terminus with, respectively, mCherry or hrGFP (humanized Renilla green fluorescent protein).
View Article and Find Full Text PDFArticle Synopsis
- Subcellular distribution of signaling components is crucial for regulating cellular signal transduction and molecular interactions.
- Understanding how such distributions affect signaling activity and what drives them is important for grasping the full picture of signal transduction.
- In primary murine T cells, actin dynamics play a key role in controlling signaling localization at the T cell and antigen presenting cell (APC) interface, with research showing that interference with actin dynamics impacts T cell activation and calcium signaling.
Objective: To determine the tissue-restricted expression pattern of tyrosinase mRNA in canine and equine melanocytic tumors and relative tyrosinase and major histocompatibility complex (MHC) I mRNA expression in variants of melanocytic tumors.
Sample: 39 canine and 8 equine tumor samples and 10 canine and 6 equine normal tissue samples.
Procedures: RNA was isolated from formalin-fixed, paraffin-embedded tissues.