Publications by authors named "A Thurm"
Article Synopsis
- The PECOS study is investigating the long-term effects of pediatric SARS-CoV-2 infections by comparing infected and uninfected participants aged 0-21.
- Participants were assessed on various health metrics, including symptom reports, physical exams, and psychological surveys, revealing that those infected reported significantly worse outcomes in several health domains.
- The findings indicate that SARS-CoV-2 infection could negatively impact children's overall quality of life, highlighting the importance of ongoing research to understand these long-term effects.
The binding of multiple transcription factors (TFs) to genomic enhancers drives gene expression in mammalian cells. However, the molecular details that link enhancer sequence to TF binding, promoter state and transcription levels remain unclear. Here we applied single-molecule footprinting to measure the simultaneous occupancy of TFs, nucleosomes and other regulatory proteins on engineered enhancer-promoter constructs with variable numbers of TF binding sites for both a synthetic TF and an endogenous TF involved in the type I interferon response.
View Article and Find Full Text PDFControlling gene expression and chromatin state via the recruitment of transcriptional effector proteins to specific genetic loci has advanced the potential of mammalian synthetic biology, but is still hindered by the challenge of delivering large chromatin regulators. Here, we develop a new method for generating small nanobodies against human chromatin regulators that can repress or activate gene expression. We start with a large and diverse nanobody library and perform enrichment against chromatin regulatory complexes using yeast display, followed by high-throughput pooled selection for transcriptional control when recruited to a reporter in human cells.
View Article and Find Full Text PDFArticle Synopsis
- Creatine transporter (CTD) and guanidinoacetate methyltransferase (GAMT) deficiencies cause serious brain issues like intellectual disabilities and seizures, with no effective treatment for CTD and a strict diet plus supplements needed for GAMT.
- A core outcome set (COS) has been developed in collaboration with caregivers and health professionals to determine key outcomes for assessing CTD and GAMT in clinical trials, including factors like cognitive functioning and emotional regulation.
- This COS aims to prioritize patient and caregiver perspectives to improve the drug development process, enhance trial comparability, reduce bias, and optimize resource use in research for these conditions.
Article Synopsis
- Histone H3.3 is often mutated in tumors, particularly the K36M mutation, which is commonly found in chondroblastomas, affecting the way genes are expressed.
- The study investigates how the H3.3K36M mutation influences gene silencing and epigenetic memory by using a synthetic reporter system and analyzing changes in histone modifications.
- Findings suggest that the K36M mutation reduces epigenetic memory and alters methylation patterns, highlighting the importance of the H3K36 methylation pathway in maintaining stable epigenetic memory, which could inform future cancer research and therapeutic approaches.