The saponin bomb: a nucleolar-localized β-glucosidase hydrolyzes triterpene saponins in Medicago truncatula.

Plants often protect themselves from their own bioactive defense metabolites by storing them in less active forms. Consequently, plants also need systems allowing correct spatiotemporal reactivation of such metabolites, for instance under pathogen or herbivore attack. Via co-expression analysis with public transcriptomes, we determined that the model legume Medicago truncatula has evolved a two-component system composed of a β-glucosidase, denominated G1, and triterpene saponins, which are physically separated from each other in intact cells.

Nematicidal Potential of Sulla ( L.) against the Root-Knot Nematode .

The content of nematicidal metabolites such as saponins, flavonoids and tannins in sulla ( L.) suggests its potential nematicidal activity. In this study, the biocidal activity of 62.

Variability in the Essential Oil Composition of Achillea erba-rotta subsp. moschata (Wulfen) I. Richardson Growing in the Eastern Italian Alps.

Achillea erba-rotta subsp. moschata (Wulfen) I. Richardson is endemic of the Central Alps and distributed in several Italian Regions, where is used for its medicinal and aromatic properties.

The Antioxidant Activity of a Commercial and a Fractionated Phycocyanin on Human Skin Cells In Vitro.

The protective effects for cells against chemical and UVA stress of a commercial phycocyanin (PC) for food use and a PC extracted from Arthrospira platensis (Spirulina) in phosphate buffer were assessed. The purity of the commercial PC, spectrophotometrically estimated as A620/A280 and confirmed by HPLC, was higher than that of the fractionated PC (2.0 vs.

CRISPR/Cas9-Mediated Targeted Mutagenesis of Modulates the Triterpene Saponin Biosynthesis in .

In the genus, triterpene saponins are a group of bioactive compounds extensively studied for their different biological and pharmaceutical properties. In this work, the CRISPR/Cas9-based approach with two single-site guide RNAs was used in (barrel medic) to knock-out the and genes, which are responsible for the biosynthesis of soyasapogenol B, the most abundant soyasapogenol in spp. No transgenic plants carrying mutations in the target gene were recovered while fifty-two putative mutant plant lines were obtained following -mediated transformation.