Low Voltage Electron Microscopy: An Emerging Tool for AAV Characterization.

Transmission electron microscopy has become a standard characterization tool for adeno-associated virus-based gene therapy products. However, cost and expertise requirements place in-house traditional transmission electron microscope systems out of reach for many companies in the field. Recently developed low voltage electron microscopes can fulfill many of the needs for adeno-associated virus characterization at a fraction of the cost.

Systematic review of musculoskeletal ultrasound learning methodologies.

Introduction/purpose: As the frequency and morbidity of musculoskeletal (MSK) disorders increase, the use of MSK ultrasound has become an important diagnostic and procedural tool. This has amplified the importance of quality MSK ultrasound training for medical professionals. This systematic review evaluated and compared peer-reviewed journal articles on learning methodologies for MSK ultrasound training.

Heterogeneity in definitions of surgical site infection after cranial surgery limits the validity of research findings in neurosurgery: a systematic review.

Surgical site infections after cranial surgery (SSI-CRAN) are serious adverse events considering the vicinity of the wound to the central nervous system. Variability in outcome definitions can hinder the ability to produce reliable evidence. This systematic review aimed to investigate whether there is variation in SSI-CRAN definitions across studies and its impact on the identification of effective treatments for patients after cranial surgery.

The Effects of Gender on Mesenchymal Stromal Cell (MSC) Proliferation and Differentiation In Vitro: A Systematic Review.

Mesenchymal stromal cells (MSCs) have the potential for novel treatments of several musculoskeletal conditions due to their ability to differentiate into several cell lineages including chondrocytes, adipocytes and osteocytes. Researchers are exploring whether this could be utilized for novel therapies for joint afflictions. The role of gender in the ability of MSCs to differentiate and proliferate into different cells has not been clearly defined.

Interlaboratory Measurement of Adeno-Associated Virus: Comparative Quantification of Full and Empty Capsids.

Recombinant adeno-associated virus (AAV) is one of the main viral vector-based gene therapy platforms. AAV is a virus consisting of a ≈25 nm diameter capsid with a ≈4.7 kb cargo capacity.