[Evaluation of significance of Borrelia garinii spirochete recombinant protein DbpB for serodiagnostics of ixodes tick-borne borreliosis].

Aim: Obtaining recombinant protein DbpB of West Siberia Borrelia garinii 20047 isolate and evaluation of its antigen activity for the possible use in serodiagnostics of ixodes tick-borne borreliosis (ITB).

Materials And Methods: Coding region of dbpB gene of novosibirsk B. garinii 20047 isolate was amplified by PCR and cloned as part of expressing pETm vector in Escherichia coli BL21 (DE3) strain cells.

[Immunochemical analysis of recombinant protein FlaA of Western Siberian Borrelia garinii isolate].

Aim: Study of the ability of Western Siberian Borrelia garinii 20047 isolate recombinant protein FlaA to react with sera antibodies of ixodes tick borreliosis patients.

Materials And Methods: Recombinant antigen FlaA, sera of ixodes tick borreliosis patients, genetic engineering methods, solid phase EIA, and parametric and nonparametric statistical methods were used in the study.

Results: Recombinant form of mature flagellar protein FlaA of B.

[Enzyme immunoassay-based analysis of OspC recombinant proteins from Borrelia garinii and Borrelia afzelii isolated in West Siberia].

Aim: To study the ability of OspC recombinant proteins from Borrelia garinii and Borrelia afzelii isolated in West Siberia to interact with serum antibodies from patients with tick-borne borreliosis (TBB).

Materials And Methods: Recombinant antigens OspC B. garinii and OspC B.

[Characteristics of wheat powdery mildew growth along and across the longitudinal axis of a leaf under the action of exogenous zeatin].

Scanning electronic microscopy was used to investigate the regularities of growth direction of infectious structures and colonies of the agent of powdery mildew of wheat Erysiphe graminis f. sp. tritici.

[Influence of exogenous cytokinins on dynamics of development and differentiation of infection structure of the causal organism of wheat powdery mildew].

Using methods of light and electron scanning microscopy, development and infection structures differentiation of the causal organism of wheat powdery mildew, Erysiphe graminis DC. f. sp.