Nanopore sequencing has shown the potential to democratize genomic pathogen surveillance due to its ease of use and low entry cost. However, recent genotyping studies showed discrepant results compared to gold-standard short-read sequencing. Furthermore, although essential for widespread application, the reproducibility of nanopore-only genotyping remains largely unresolved.
View Article and Find Full Text PDFIntroduction: is an ubiquitous foodborne pathogen that represents a serious threat to public health and the food industry.
Methods: In this study Whole Genome Sequencing (WGS) was used to characterize 160 isolates obtained from 22,593 different food sources in Montenegro during the years 2014-2022.
Results: Isolates belonged to 21 different clonal complexes (CCs), 22 sequence types (STs) and 73 core genome multilocus sequence types (cgMLST) revealing a high diversity.
Background: Listeria monocytogenes is a bacterial pathogen known for causing listeriosis, a foodborne illness with a wide spectrum of clinical presentations ranging from mild gastroenteritis to severe invasive disease, particularly affecting immunocompromised individuals, pregnant women, newborns, and the elderly. Successful treatment of patients with recurring listeria episodes due to colonised foreign material is often challenging, typically requiring a combination of antimicrobial treatment and surgical removal.
Case Presentation: Here, we present a particularly complex case of chronic invasive listeriosis with a total of six relapses.