Dynamic interplay of autophagy and membrane repair during Mycobacterium tuberculosis Infection.

Autophagy plays a crucial role in the host response to Mycobacterium tuberculosis (Mtb) infection, yet the dynamics and regulation of autophagy induction on Mtb-containing vacuoles (MCVs) remain only partially understood. We employed time-lapse confocal microscopy to investigate the recruitment of LC3B (LC3), a key autophagy marker, to MCVs at the single cell level with our newly developed workflow for single cell and single MCV tracking and fluorescence quantification. We show that approximately 70% of MCVs exhibited LC3 recruitment but that was lost in about 40% of those MCVs.

Anticipated facilitators and barriers for long-acting injectable antiretrovirals as HIV treatment and prevention in Vietnam: a qualitative study among healthcare workers.

Background: Long-acting injectable antiretrovirals (LAI-ARVs) for HIV prevention and treatment have been demonstrated in clinical trials to be non-inferior to daily oral medications, providing an additional option to help users overcome the challenges of daily adherence. Approval and implementation of these regimens in low- and middle-income settings have been limited.

Method: This study describes the anticipated barriers and facilitators to implementing LAI-ARVs in Vietnam to inform future roll-out.

Mapping of high-resolution daily particulate matter (PM) concentration at the city level through a machine learning-based downscaling approach.

PM pollution is a major global concern, especially in Vietnam, due to its harmful effects on health and the environment. Monitoring local PM levels is crucial for assessing air quality. However, Vietnam's state-of-the-art (SOTA) dataset with a 3 km resolution needs to be revised to depict spatial variation in smaller regions accurately.