A chemically defined culture medium containing Rho kinase inhibitor Y-27632 for the fabrication of stratified squamous epithelial cell grafts.

With the development of a culture method for stratified squamous epithelial cells, tissue-engineered epithelial cell sheets have been successfully applied as clinical cell grafts. However, the implementation of these cell sheets without the use of any animal-derived materials is highly desirable. In this study, Rho-associated protein kinase inhibitor Y-27632 was used to develop a chemically defined culture medium for the fabrication of stratified epithelial cell grafts consisting of human epidermal and oral keratinocytes, and the proliferation activity, cell morphology, and gene expressions of the keratinocytes were analyzed.

Seroprevalence of tularemia in rural Azerbaijan.

A representative, two-stage probability sampling design was used to select 40 villages in northern Azerbaijan with populations of <500 people to screen for evidence of prior infection with Francisella tularensis. Informed consent was provided, and samples were obtained from 796 volunteers and tested for the presence of immunoglobulin G antibodies using enzyme-linked immunosorbent assay. F.

Under-utilization of health care services for infectious diseases syndromes in rural Azerbaijan: a cross-sectional study.

Background: Infectious diseases present a potentially substantial yet undefined burden on the health of the adult Azerbaijani population. Efforts to quantify this burden in Azerbaijan are currently based almost exclusively on passive disease surveillance, and therefore hinge on the health utilization practices of the population. Understanding the prevalence of infectious syndromes and health utilization practices is paramount to disease surveillance, public health planning, and health care system reform.

[Features of treating patients with combined damage to the orbit and eye in the late period in peacetime and war conditions].

Analysing the diagnosis and treatment of 100 patients with combined injuries of the organ of vision and orbital zone of the face provided clinical features of lesions in peace-time or battle accidents. Battle accidents were found to be prognostically worse for visual functions. The use of immunotherapy for inflammatory complications of a traumatic process is demonstrated to be advisable.