Collagen orientation probed by polarized Raman spectra can serve as differential diagnosis indicator between different grades of meniscus degeneration.

The purpose of the present study was to analyze normal and degenerated menisci with Raman methodology on thin sections of formalin fixed paraffin embedding tissues and to correlate the Raman findings with the grade of meniscus degeneration. Menisci (n = 27) were removed from human knee joints after total knee replacement or meniscectomy. Following routine histopathological analysis to determine the grade of meniscal lesions obtained from healthy and degenerated formaline fixed paraffin embedded (FFPE) meniscal sections, Raman polarization approach was applied to evaluate the orientation of collagen fibrils in different levels of the same 5 μm thick FFPE meniscal tissue sections, used for histopathological assessment.

Modeling the Solvent Extraction of Cadmium(II) from Aqueous Chloride Solutions by 2-pyridyl Ketoximes: A Coordination Chemistry Approach.

The goal of this work is to model the nature of the chemical species [CdCl(extractant)] that are formed during the solvent (or liquid-liquid) extraction of the toxic cadmium(II) from chloride-containing aqueous media using hydrophobic 2-pyridyl ketoximes as extractants. Our coordination chemistry approach involves the study of the reactions between cadmium(II) chloride dihydrate and phenyl 2-pyridyl ketoxime (phpaoH) in water-containing acetone. The reactions have provided access to complexes [CdCl(phpaoH)]∙HO (∙HO) and {[CdCl(phpaoH)]} (); the solid-state structures of which have been determined by single-crystal X-ray crystallography.

Quantitative analysis via Surface Enhanced Raman Scattering from Ag nano-colloids utilizing an oscillating cell and right-angle collection geometry.

We examine the application of an oscillating cell in combination with right-angle Raman scattered light collection geometry for quantitative Surface Enhanced (Resonance) Raman Scattering (SER(R)S) measurements from nano-colloidal noble metal solutions. This excitation/collection Raman configuration allows specific SERS and SERRS signatures of aqueous solutions of mitoxantrone, an antitumor drug, to be easily resolved at (sub)-ng/mL and (sub)-pg/mL concentration levels. A partial least-squares (PLS) chemometric algorithm was applied to predict the concentration of 25 microL of aqueous solutions of mitoxantrone added in 0.