Application of high-performance size exclusion chromatography to the determination of erythropoietin in pharmaceutical preparations.

High performance size exclusion chromatography with fluorimetric detection for the determination of erythropoietin in pharmaceutical preparations has been developed. The applied chromatographic system has been enabled a quick estimation of the identity and contents of the studied compound. The method was validated and showed good validation data in terms of linearity, precision and repeatability.

Determination of the content of desmopressin in pharmaceutical preparations by HPLC and validation of the method.

The aim of this study was to apply high performance liquid chromatography to the determination of content of desmopressin in pharmaceutical preparations and validation of the method. The satisfactory results have been obtained using a column Luna C 8.5 microm, 100 x 4.

HPLC method for the determination of oxytocin in pharmaceutical dosage form and comparison with biological method.

Conditions have been established for the determination of oxytocin by the HPLC method; the method has been validated. The results of HPLC determinations are compared with those obtained by the biological method.

Determination of protamine sulphate in drug formulations using high performance liquid chromatography.

Protamine sulphate, which has the property of neutralising heparin, is determined in pharmaceutical formulations using spectrophotometric (BP 1995) or biochemical methods (USP XXIII 1995). Accuracy of these methods is not very high. We applied the HPLC technique for the assay of protamine sulphate in a gel formulation.

Polarographic and absorptive stripping voltammetric determination of mitoxantrone, emodin and chrysarobin using mercury and glassy carbon electrodes.

Polarographic and voltammetric methods were used to study drugs of anthrone derivatives: mitoxandrone (antineoplastic), emodin (cathartic) and chrysarobin (antipsoriatic) on mercury and glassy carbon electrodes. The surface-active properties were exploited for developing a highly sensitive adsorptive stripping voltammetric method for determination of trace amounts of these compounds. Effective pre-concentration was obtained at glassy carbon electrode, with the surface species being measured via its reduction or oxidation, respectively.