Rapid assay of dinitrophenyl derivative of taurine by high-performance liquid chromatography.

A rapid and simple method for the determination of taurine (2-aminoethanesulphonic acid) in complex samples is described. It is based on the HPLC separation of the dinitrophenyl (DNP) derivative of taurine. The reaction conditions are selected to allow complete derivatization of taurine within 15 min.

[Carbon isotope fractionation by aerobic heterotrophic microorganisms].

The isotope effects of carbon were studied in auxotrophic mutants of the following aerobic microorganisms: Escherichia coli, Corynebacterium, Bacillus subtilis, Brevibacterium and Micrococcus glutamicus. Intramolecular isotope heterogeneity was found in position of the total carbon differed from that of the carbon of the carboxyl in the amino acid. In the lysine released by Corynebacterium grown on acetate, the carboxyl carbon is enriched with 13C by 8%o comparing with the total carbon of the amino acid; in the lysine liberated by Brevibacterium flavum, the carboxylic carbon is enriched with 13C by 3.