Penetration of mixed micelles into the epidermis: effect of mixing sodium dodecyl sulfate with dodecyl hexa(ethylene oxide).

The penetration of the anionic surfactant sodium dodecyl sulfate (SDS) into the epidermis from contacting solutions of SDS and the nonionic surfactant dodecyl hexa(ethylene oxide) (C(12)E(6)) was measured for three SDS concentrations (25 mM, 50 mM, and 100 mM) and three SDS solution compositions (1, 0.83, and 0.50).

Use of high density cultures of Escherichia coli for high level production of recombinant Pseudomonas aeruginosa exotoxin A.

An efficient fermentation method for the production of two modified recombinant Pseudomonas aeruginosa exotoxin As cloned in Escherichia coli BL21(lambda DE3) was developed. Cell densities of 16-30 g dry weight/1 were found to be most suitable for the induction of protein synthesis, which was under the isopropyl beta-D-thiogalactopyranoside (IPTG)-inducible T7 expression system. A concentration of 0.