Protocol to expand and CRISPR-Cas9 genomic edit murine MAIT cells for subsequent in vivo studies.

Generating knockout mice for target molecules in specific T cell populations, without subset-specific promoters, is time-consuming and costly. Here, we describe steps for enriching mucosal-associated invariant T cells from the thymus, expanding them in vitro and performing a CRISPR-Cas9 knockout. We then detail procedure for injecting the knockout cells into wounded Cd3ε mice and characterizing them in the skin.

A Hybrid Stacked Restricted Boltzmann Machine with Sobel Directional Patterns for Melanoma Prediction in Colored Skin Images.

Melanoma, a kind of skin cancer that is very risky, is distinguished by uncontrolled cell multiplication. Melanoma detection is of the utmost significance in clinical practice because of the atypical border structure and the numerous types of tissue it can involve. The identification of melanoma is still a challenging process for color images, despite the fact that numerous approaches have been proposed in the research that has been done.

Role of MR1-driven signals and amphiregulin on the recruitment and repair function of MAIT cells during skin wound healing.

Tissue repair processes maintain proper organ function following mechanical or infection-related damage. In addition to antibacterial properties, mucosal associated invariant T (MAIT) cells express a tissue repair transcriptomic program and promote skin wound healing when expanded. Herein, we use a human-like mouse model of full-thickness skin excision to assess the underlying mechanisms of MAIT cell tissue repair function.

Exploring Users' Experiences With a Quick-Response Chatbot Within a Popular Smoking Cessation Smartphone App: Semistructured Interview Study.

Background: Engagement with smartphone apps for smoking cessation tends to be low. Chatbots (ie, software that enables conversations with users) offer a promising means of increasing engagement.

Objective: We aimed to explore smokers' experiences with a quick-response chatbot (Quit Coach) implemented within a popular smoking cessation app and identify factors that influence users' engagement with Quit Coach.