Publications by authors named "A Scipioni"
Article Synopsis
- Many agrochemicals are chiral and often sold as mixtures, but inactive isomers can be toxic to non-target organisms, highlighting the need for analyzing specific isomers.
- Researchers developed an enantioselective high-performance liquid chromatography-mass spectrometry method to detect nine chiral pesticides in urine, utilizing two solid-phase extraction techniques (GCB and BP) for optimal recovery rates.
- The method was validated following FDA guidelines and demonstrated effective separation and detection of pesticide enantiomers, allowing for analysis of exposure in workers handling these chemicals.
Specificity in protein-DNA recognition arises from the synergy of several factors that stem from the structural and chemical signatures encoded within the targeted DNA molecule. Here, we deciphered the nature of the interactions driving DNA recognition and binding by the bacterial transcription factor PdxR, a member of the MocR family responsible for the regulation of pyridoxal 5'-phosphate (PLP) biosynthesis. Single particle cryo-EM performed on the PLP-PdxR bound to its target DNA enabled the isolation of three conformers of the complex, which may be considered as snapshots of the binding process.
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- There is increasing interest in using saliva microRNAs as non-invasive biomarkers for diseases, but varying experimental designs greatly affect miRNA expression and study reliability.
- Differences in technical methods, individual variations, and the lack of standardized control miRNAs pose challenges for research accuracy.
- A large study assessed how factors like saliva collection methods and RNA stabilizers impact miRNA variability, finding that RNA stabilizers significantly affect measurements and identifying certain salivary miRNAs that could serve as reliable controls.
Anthracycline doxorubicin hydrochloride (DX) is a positively charged fluorescent drug, which in water self-associates into non-fluorescent antiparallel dimers upon increasing concentration and/or ionic strength. The positive charge of DX allows for complexation with negatively charged polymers and drug carriers. The fluorescence of DX following complexation with polyanion polystyrene sulfonate (PSS) is studied here.
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