Pathogen-Specific Electrochemical Real-Time LAMP Detection Using Universal Solid-Phase Probes on Carbon Electrodes.

Epidemic infections and spreading antibiotic resistance require diagnostic tests that can be rapidly adopted. To reduce the usually time-consuming adaptation of molecular diagnostic tests to changing targets, we propose the novel approach of a repurposable sensing electrode functionalization with a universal, target-independent oligonucleotide probe. In the liquid phase covering the electrode, the target sequence is amplified by MD LAMP (mediator-displacement loop-mediated isothermal amplification) releasing a generic methylene blue-labeled mediator, which specifically hybridizes to the solid-phase probe.

Enhancing Antifungal Drug Discovery Through Co-Culture with Antarctic Strain CBMAI 1855.

Fungal infections pose a growing public health threat, creating an urgent clinical need for new antifungals. Natural products (NPs) from organisms in extreme environments are a promising source for novel drugs. CBMAI 1855 exhibited significant potential in this regard.

Lung ultrasound score and diaphragm ultrasound in weaning from mechanical ventilation: are they different in patients with and without COVID-19?

Objective: To compare pre-extubation physiological characteristics and ultrasound variables between patients intubated for COVID-19 compared to a clinical population and those intubated for other reasons.

Methods: This was a secondary analysis of a prospective cohort study of patients undergoing invasive mechanical ventilation (IMV) for more than 48 h. Patients were divided into two groups: those intubated for COVID-19-induced ARDS and those intubated for other clinical reasons.

Prediction of vertebral failure under general loadings of compression, flexion, extension, and side-bending.

Bone pathologies such as osteoporosis and metastasis can significantly compromise the load-bearing capacity of the spinal column, increasing the risk of vertebral fractures, some of which may occur during routine physical activities. Currently, there is no clinical tool that accurately assesses the risk of vertebral fractures associated with these activities in osteoporotic and metastatic spines. In this paper, we develop and validate a quantitative computed tomography-based finite element analysis (QCT/FEA) method to predict vertebral fractures under general load conditions that simulate flexion, extension, and side-bending movements, reflecting the body's activities under various scenarios.