Radiographic and clinical results after surgically treated tibial plateau fractures at three and twenty two years postsurgery.

Purpose: We examined the development of osteoarthritis (OA) and post-traumatic bone loss after surgery for tibial plateau fractures (TPF).

Methods: Patients who had participated in previous follow-up (FU) examinations after TPF and primary reduction and internal fixation were re-evaluated. At the first FU, a median of three years after the accident (short-term FU), the patients underwent functional assessments and standardised X-rays to grade radiological OA and post-traumatic bone loss.

The steady-state mRNA levels for thylakoid proteins exhibit coordinate diurnal regulation.

Steady-state mRNA levels for thylakoid proteins were analysed in spinach cotyledons under diurnally changing light conditions. Most fluctuate considerably throughout the day, while the levels of others show only low amplitude or no oscillation. Levels of mRNAs coding for proteins that belong to the same multiprotein complex generally oscillate in parallel and exhibit maxima that are specific for that complex: mRNAs for photosystem I proteins appear prior to those for photosystem II polypeptides and these again prior to mRNAs for the three polypeptides constituting the oxygen-evolving complex.

Response of a nitrite-reductase 3.1-kilobase upstream regulatory sequence from spinach to nitrate and light in transgenic tobacco.

In the present study the question was addressed of whether the nitrite-reductase (NIR-)promoter from spinach (Spinacia oleracea L.), fused to a reporter gene (bacterial β-glucuronidase, GUS) and introduced into tobacco (Nicotiana tabacum L.) responds to nitrate and light in accordance with spinach (donor) or in accordance with tobacco (host).

Isolation of functional RNA from plant tissues rich in phenolic compounds.

A method for the isolation of RNA from different tissues of trees (seedlings, saplings, and adult trees) is described. Using this procedure it is possible to remove large amounts of disturbing polyphenolic compounds from nucleic acids. The method involves an acetone treatment of the freeze-dried and powdered plant material, the use of high salt concentrations in the extraction buffer and an aqueous two-phase system.

Isolation of the spinach nitrite reductase gene promoter which confers nitrate inducibility on GUS gene expression in transgenic tobacco.

Nitrite reductase is the second enzyme in the nitrate assimilatory pathway. The transcription of this gene is regulated by nitrate as well as a variety of other environmental and developmental factors. Genomic clones containing the entire nitrite reductase gene have been isolated from a spinach genomic library and sequenced.