Publications by authors named "A Scarborough"
Background: Ang-II (angiotensin II) impairs the function of the antihypertensive enzyme ACE2 (angiotensin-converting enzyme 2) by promoting its internalization, ubiquitination, and degradation, thus contributing to hypertension. However, few ACE2 ubiquitination partners have been identified, and their role in hypertension remains unknown.
Methods: Proteomics and bioinformatic analyses were used to identify ACE2 ubiquitination partners in the brain, heart, and kidney of hypertensive C57BL6/J mice of both sexes.
Article Synopsis
- The study investigated the impact of cardiopulmonary resuscitation (CPR) on brain and skin oxygen levels in pigs experiencing severe hemorrhagic shock, a condition where blood pressure drops critically.
- Using a controlled experiment with 12 swine, results indicated that while CPR increased systolic blood pressure, it did not improve overall tissue oxygenation and, in fact, reduced skin perfusion significantly.
- The findings support previous research suggesting that CPR may not benefit organ oxygenation during hemorrhagic shock and could even have harmful effects.
Article Synopsis
- Angiotensin-II negatively affects ACE2, an important antihypertensive enzyme, by promoting its breakdown, contributing to high blood pressure.
- Researchers identified UBR1 as a key protein that helps in the ubiquitination of ACE2, particularly heightened in hypertensive conditions and showing sex-specific regulation by testosterone.
- Silencing UBR1 in mice led to increased ACE2 levels and a temporary drop in blood pressure, suggesting that targeting UBR1 and related proteins could be a new treatment strategy for hypertension.
Premise: Traditional moist chamber cultures (MCs) prepared in aseptic laboratory environments using sterile Petri dishes are commonly used to quantify the microbiota of rough-bark tree species and woody vines. MCs are typically expensive and may be difficult to make, so a less expensive option made from easily available supplies was developed. These cost-friendly MCs were compared with standard laboratory methods to demonstrate their efficacy.
View Article and Find Full Text PDFTeaching during a surgical resuscitation can be difficult due to the infrequency of these events. Furthermore, when these events do occur, the trainee can experience cognitive overload and an overwhelming amount of stress, thereby impairing the learning process. The emergent nature of these scenarios can make it difficult for the surgical educator to adequately teach.
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