Leveraging automated time-lapse microscopy coupled with deep learning to automate colony forming assay.

Introduction: The colony forming assay (CFA) stands as a cornerstone technique for evaluating the clonal expansion ability of single cancer cells and is crucial for assessing drug efficacy. However, traditional CFAs rely on labor-intensive, endpoint manual counting, offering limited insights into the dynamic effects of treatment. To overcome these limitations, we developed an Artificial Intelligence (AI)-assisted automated CFA combining time-lapse microscopy for real-time tracking of colony formation.

Investigating the thin film growth of [Ni(Hvanox)] by microscopic and spectroscopic techniques.

We have investigated [Ni(Hvanox)] (Hvanox = -vanillinoxime), a square-planar Ni(ii) complex, for the preparation of thin films using organic molecule evaporation. Low pressure experiments to prepare thin films were conducted at temperatures between 120-150 °C and thin films of increasing thicknesses [Ni(Hvanox)] (16-336 nm) have been prepared on various substrates and been analyzed by microscopic and spectroscopic methods. Scanning electron microscopy (SEM), atomic force microscopy (AFM) and transmission electron microscopy (TEM) were used to reveal a rough surface morphology which exhibits a dense arrangement of elongated, rod and needle-like nanocrystals with random orientations.

Ionic gradients in flow to control transport of emissive ions.

Concentration gradients of simple salts in microfluidic channels control the transport of a common photoredox catalyst.

Successful Surgical Repair of Complete Pentalogy of Cantrell.

Pentalogy of Cantrell (PC) presents a distinctive challenge for clinicians and surgeons. In this case report, we have discussed the presentation, management, and literature review of a case of PC in a 17-month-old female child. The child was successfully managed with single-stage operation by a multidisciplinary team without any postoperative complications.