Publications by authors named "A Saliez"
Background: In vivo studies have highlighted allogeneic mesenchymal stem-cell (MSC) immunogenicity. We investigated in vitro MSC-immunosuppressive drugs interaction and further tested in vivo the humoral response to intracardiac allogeneic MSC transplantation in a mini-swine model receiving a short course of immunosuppression.
Methods: For in vitro experiments, long-term culture MSCs were used.
Ras oncoproteins are probably implicated in normal and malignant cell growth in various organs. Inhibition of Ras interferes with cell proliferation of non-hepatic cells in vitro and in vivo. A potential role for Ras in normal and malignant hepatocyte proliferation prompted us to evaluate the impact of Ras inhibition by FTS (S-farnesylthiosalicylic acid) on hepatocyte proliferation in vitro in the human hepatic tumour cell line HepG2 and in vivo after PH (partial hepatectomy) in rats.
View Article and Find Full Text PDFBackground: In vitro, mesenchymal stem cells (MSCs) have demonstrated a low immunogenic profile. In this study, we tested the immune response to allogeneic MSCs in immunocompetent swines both in vitro and in vivo.
Methods: Major histocompatibility complex-controlled swine leukocyte antigen (SLA) and SLA were used as donor and recipient, respectively.
Background: In the near future, adult porcine islets of Langerhans appear as an unlimited source of insulin-producing cells which could play a major role for treating diabetes mellitus. There is, however, an obvious lack of pre-clinical results and data in the pig-to-primate model. One of the main hurdles of this model is certainly related to the difficulty of reproducing regularly successful porcine islet isolation.
View Article and Find Full Text PDFArticle Synopsis
- The study investigates the long-term compatibility of alginate-encapsulated pig islets in primates, given the immune response challenges associated with xenotransplantation.
- Results show that well-encapsulated pig islets had a notable survival rate of up to 6 months post-transplant in primates, while poorly encapsulated or non-encapsulated islets were quickly destroyed.
- Despite an immune response, optimal encapsulation allowed for some pig islet functionality, indicated by insulin production and responsiveness to glucose.