Enhancement of mono-acylated MEL-D production in an acyltransferase gene-deleted strain of by supplementation with di-acylated MEL-B in culture medium.

Mannosylerythritol lipids (MELs) are glycolipid biosurfactants produced by various yeasts. MEL producers produce mainly di-acylated MELs (consisting of two fatty acid chains). Among them, is a di-acylated MEL-B (d-MEL-B) producer.

QcrC is a potential target for antibody therapy and vaccination to control infection by suppressing its energy metabolism.

Introduction: spp. are a public health concern, yet there is still no effective vaccine or medicine available.

Methods: Here, we developed a -specific antibody and found that it targeted a menaquinol cytochrome reductase complex QcrC.

Characterization of a KU70-disrupted strain of the mannosylerythritol lipid-producing yeast Pseudozyma tsukubaensis constructed by a marker recycling system.

The basidiomycetous yeast Pseudozyma tsukubaensis is known as an industrial mannosylerythritol lipid producer. In this study, the PtURA5 marker gene was deleted by homologous recombination. Using the PtURA5-deleted mutant as a host strain, we obtained a derivative disrupted for the PtKU70 gene, a putative ortholog of the KU70 gene encoding a protein involved in the nonhomologous end-joining pathway of DNA repair.

The omega-3 postbiotic -10--15-octadecadienoic acid attenuates contact hypersensitivity in mice through downregulation of vascular endothelial growth factor A.

Intestinal bacteria metabolize dietary substances to produce bioactive postbiotics, among which some are recognized for their role in promoting host health. We here explored the postbiotic potential of two omega-3 α-linolenic acid-derived metabolites: -10--15-octadecadienoic acid (t10,c15-18:2) and -9--15-octadecadienoic acid (c9,c15-18:2). Dietary intake of lipids rich in omega-3 α-linolenic acid elevated levels of t10,c15-18:2 and c9,c15-18:2 in the serum and feces of mice, an effect dependent on the presence of intestinal bacteria.

RNA-Based Anti-Inflammatory Effects of Membrane Vesicles Derived from .

Bacteria generally release extracellular membrane vesicles (MVs), which are nanoparticles that play important roles in bacterial-bacterial and bacterial-host communication. As probiotics, lactic acid bacteria provide diverse health benefits to their hosts. In this study, we found that the Gram-positive lactic acid bacteria subsp.