A new concept of biocatalytic synthesis of acrylic monomers for obtaining water-soluble acrylic heteropolymers.

strains were designed as model biocatalysts (BCs) for the production of acrylic acid and mixtures of acrylic monomers consisting of acrylamide, acrylic acid, and N-alkylacrylamide (N-isopropylacrylamide). To obtain BC strains, we used, among other approaches, adaptive laboratory evolution (ALE), based on the use of the metabolic pathway of amide utilization. Whole genome sequencing of the strains obtained after ALE, as well as subsequent targeted gene disruption, identified candidate genes for three new amidases that are promising for the development of BCs for the production of acrylic acid from acrylamide.

Rational metabolic engineering of Corynebacterium glutamicum to create a producer of L-valine.

L-Valine is one of the nine amino acids that cannot be synthesized de novo by higher organisms and must come from food. This amino acid not only serves as a building block for proteins, but also regulates protein and energy metabolism and participates in neurotransmission. L-Valine is used in the food and pharmaceutical industries, medicine and cosmetics, but primarily as an animal feed additive.

: sequences of genetic parts, analysis of their functionality, and development prospects as a molecular biology platform.

bacteria are a fast-growing platform for biocatalysis, biodegradation, and biosynthesis, but not a platform for molecular biology. That is, are not convenient for genetic engineering. One major issue for the engineering of is the absence of a publicly available, curated, and commented collection of sequences of genetic parts that are functional in biotechnologically relevant species of (, , , and ).

Draft Genome Sequence of Rhodococcus qingshengii (Formerly ) TA37, a First-Generation Biocatalyst for Synthesis of Functionalized Acrylamides.

We describe here the 7.0-Mb draft genome sequence of Rhodococcus qingshengii strain TA37, which was obtained from samples of nitrile-contaminated soil collected in the Saratov Region (Russian Federation). This genomic resource will support the further development of biocatalysts for the inexpensive and green production of acrylic monomers.

Complete Genome Sequence of sp. Strain M8, a Platform Strain for Acrylic Monomer Production.

We report a 6.27-Mbp complete genome of sp. strain M8, an originally discovered strain that is now under investigation for production of acrylic monomers.