Single-Nucleus and Spatial Transcriptome Profiling Delineates the Multicellular Ecosystem in Hepatocellular Carcinoma After Hepatic Arterial Infusion Chemotherapy.

Hepatic arterial infusion chemotherapy (HAIC) has emerged as a promising treatment strategy for hepatocellular carcinoma (HCC), but a detailed understanding of the multicellular ecosystem after HAIC treatment is lacking. Here, we collected tumor samples from treatment-naïve primary and post-HAIC HCC, and integrated single-nucleus RNA sequencing with spatial transcriptomics to characterize the tumor ecosystem in the post-HAIC HCC. Increased fractions and enhanced cellular communication of CD4 T, CD20 B, and dendritic cell subtypes were identified in post-HAIC tumors.

Phase transitions in anisotropic turbulence.

Turbulence is a widely observed state of fluid flows, characterized by complex, nonlinear interactions between motions across a broad spectrum of length and time scales. While turbulence is ubiquitous, from teacups to planetary atmospheres, oceans, and stars, its manifestations can vary considerably between different physical systems. For instance, three-dimensional turbulent flows display a forward energy cascade from large to small scales, while in two-dimensional turbulence, energy cascades from small to large scales.

A novel automated chemiluminescent enzyme immunoassay (CLEIA) for ADAMTS13 activity enables accompanying measurements of the inhibitory autoantibodies.

Background: Thrombotic thrombocytopenic purpura (TTP) is a fatal disease caused by severe deficiency in ADAMTS13 activity. ADAMTS13 activity measurement is essential for the diagnosis of TTP, but conventional standard assays are manual and time-consuming. Automated ADAMTS13 activity assays have recently become available; however, their accuracy remains challenging.

Living Porous Ceramics for Bacteria-Regulated Gas Sensing and Carbon Capture.

Microorganisms hosted in abiotic structures have led to engineered living materials that can grow, sense, and adapt in ways that mimic biological systems. Although porous structures should favor colonization by microorganisms, they have not yet been exploited as abiotic scaffolds for the development of living materials. Here, porous ceramics are reported that are colonized by bacteria to form an engineered living material with self-regulated and genetically programmable carbon capture and gas-sensing functionalities.

Understanding paralogous epilepsy-associated GABA receptor variants: Clinical implications, mechanisms, and potential pitfalls.

Recent discoveries have revealed that genetic variants in γ-aminobutyric acid type A (GABA) receptor subunits can lead to both gain-of-function (GOF) and loss-of-function (LOF) receptors. GABA receptors, however, have a pseudosymmetrical pentameric assembly, and curiously diverse functional outcomes have been reported for certain homologous variants in paralogous genes (paralogous variants). To investigate this, we assembled a cohort of 11 individuals harboring paralogous M1 proline missense variants in , , and Seven mutations (α1, α1, β2, β3, β3, γ2, and γ2) in α1β2/3γ2 receptors were analyzed using electrophysiological examinations and molecular dynamics simulations.