Predicting modular functions and neural coding of behavior from a synaptic wiring diagram.

A long-standing goal in neuroscience is to understand how a circuit's form influences its function. Here, we reconstruct and analyze a synaptic wiring diagram of the larval zebrafish brainstem to predict key functional properties and validate them through comparison with physiological data. We identify modules of strongly connected neurons that turn out to be specialized for different behavioral functions, the control of eye and body movements.

Characterising ATP binding activity of PilB ATPase of MR-1 using a molecular modeling and simulations approach.

This study characterises the interaction between the type IV pilus assembly PilB ATPase of a versatile electroactive microbe, MR-1, and ATP using tools. PilB ATPase, which is associated with different cellular activities, is a protein subunit of type IV pili. A composite model of the protein was generated using the I-TASSER Web server and its stereochemical quality was evaluated using PROCHECK.

A low-cost optofluidic platform for the colorimetric assessment of bacterial activity in domestic wastewater.

Optofluidic chips represent a cost-effective platform for the development of miniaturized devices to perform biochemical reactions at a microscale. The dye reduction-based electron-transfer activity monitoring (DREAM) assay is a colorimetric approach that has been adopted for the rapid assessment of bacterial activity in bioreactors used in bioremediation and industrial biotechnology. A three-layered PMMA-based optofluidic chip having laser-machined microchannels coupled with a detection system comprising an LED source and a photodiode interfaced with a microcontroller for automation constituted the experimental setup.

Cyclic structure with cellular precision in a vertebrate sensorimotor neural circuit.

Neuronal wiring diagrams reconstructed by electron microscopy pose new questions about the organization of nervous systems following the time-honored tradition of cross-species comparisons. The C. elegans connectome has been conceptualized as a sensorimotor circuit that is approximately feedforward, starting from sensory neurons proceeding to interneurons and ending with motor neurons.