[The heterogeneity of the immunological phenotype and of the proliferative characteristics of the lymphoid cells in chronic B-cell lympholeukemia].

Patients with stage III and IV (according to Rai) B-cell chronic lymphocytic leukemia (B-CLL) were shown to differ in the expression of markers such as HLA-DR, RFB-1, CD-18, CD-21, activated B-lymphocyte antigen and B-lymphocyte differentiation antigen on mononuclear fraction cells (MFC). Also, differences in the level of MFC proliferation activity during a 72-hour culturing in vitro were established. Some patients with B-CLL showed an increase in lymphocyte proliferation activity in response to treatment with growth factor recombinant interleukin (IL-2) alone and in combination with phytohemagglutinin and phorbol ester.

[Ultrastructural organization of nucleoli and ribosome level in lymphocytes at different stages of maturation in patients with chronic B-cell lymphocytic leukemia].

To clarify the role of the ribosome apparatus in the differentiation and maturation, the nucleolar ultrastructure, ribosome level and maturation stages of lymphocytes and their phorbol ester-induced differentiation capacity were studied immunologically, cytochemically and electron-microscopically in 16 patients with B-chronic lymphoid leukemia (B-CLL) and in one with small lymphocyte lymphoma. Most B-CLL cases had the clones mainly consisting of cells at an intermediate maturation stage. The cases with immature and mature lymphocyte clones were observed more rarely.

[Effect of mitogenic stimulation and heat shock on protein syntheses in human T cells].

Effects of moderate (42 degrees C, 1 hour) and strong (44 degrees C, 1 hour) heat shocks on resting (TR) and phytohemagglutinin stimulated human T-cells (TP) were studied. Both treatments were shown to cause in the latter considerable fall of the level of protein synthesis, as compared to resting cells. Mitogen-stimulated cells stopped their proliferation irreversibly and part of them (approx: 40%) died after even mild shock (at 42 degrees C).

[Study of the characteristics of various hematologic diseases using monoclonal antibodies LT1, LT2 and LT7].

Monoclonal antibodies (MCA) LT1, LT2 and LT7 were characterized. It was established that MCA LT1 and LT2 (IgG1 and IgM classes, respectively) interacted with antigen 67 kD, and MCA LT7 (IgGZa)--with antigen 40 kD. MCA LT1, LT2 and LT7 were shown to identify normal and neoplastic cells of T-lymphoid type.

[Clinical characteristics and prognosis of various immunocytological subvariants of acute lymphoblastic leukemia in children].

The present investigation has demonstrated that it is advisable to specify different immunologic subvarieties of acute lymphoblastic leukemia based on the detection of superficial markers of blast cells. Acute lymphoblastic leukemia subvarieties are characterized by different clinical and hematological manifestations and differ with respect to the sensitivity to cytostatic therapy. The duration of remissions and lifespans was different in children belonging to different groups.