[Study of immunoglobulin E in sera and bronchoalveolar fluid of patients with pulmonary tuberculosis].

A highly sensitive sandwich-variety of EIA was used to measure the content of IgE in 144 sera and 59 samples of the bronchoalveolar fluid (BAF) of patients with pulmonary tuberculosis. As compared to normal persons, the content of serum IgE in the patients was significantly increased; the content of IgE in tuberculosis patients appeared as high as that in patients suffering from atopic allergy (pollenosis, bronchial asthma). The highest content of IgE was detected in patients with disseminated pulmonary tuberculosis, in the active disease phase, including those with bacterial isolation.

[Diagnostic significance of determining specific antibodies of various isotypes in patients with pulmonary tuberculosis].

The levels of antimycobacterial antibodies of three isotypes: IgG, IgA and IgM were determined in serum of 153 patients with pulmonary tuberculosis and 136 healthy donors by the solid phase enzyme immunoassay with the sonicate as the antigen. A significant and highly reliable increase in the average levels of the antimycobacterial antibodies of the IgG, IgA and IgM classes in the serum of the patients was detected as compared to the healthy persons. A significant increase (probability of 97.

[A comparison of the sorption capability and standardization of plates of different shapes for immunoenzyme analysis].

The sorption characteristics of ELISA plates manufactured by the Moscow and Leningrad plants and by some foreign firms are compared. Sorption activities and standard specifications of the plates for chemically heterogeneous antigens like ovalbumin or Shigella sonnei lipopolysaccharides and for complex antigens like Mycobacterium tuberculosis or S. sonnei ribosomal fractions have been examined.

[Determination of the concentration of immunoglobulins and other proteins in bronchoalveolar fluid of patients with various types of pulmonary pathology].

The levels of immunoglobulins and other proteins (alpha 2-MG, alpha 1-AT, C3, albumin, transferrin and lactoferrin) were studied in the BAL of 60 patients with different types of pulmonary tuberculosis, 4 patients with sarcoidosis and 7 CNPD patients. The level of most proteins in BAL of the examinees was higher than that reported for healthy subjects. The highest protein levels were noted in CNPD and sarcoidosis patients.