[Identification of cells with lowered sensitivity to cytostatic agents by the use of fluorescent dyes].

With a help of stepwise increase of vincristine concentrations in culture medium several lines of mouse myeloma X63 Ag 8.863 cells resistant to low concentrations of vincristine (6-35-fold) were selected. Rhodamine 123 stained resistant cells and wild-type cells with an equal intensity.

[Mouse cell accumulation of Colcemid and colchicine in tissue culture].

Kinetics of the uptake of 3H-colchicine and 3H-colcemid by mouse transformed L cells has been studied. The amounts of colchicine and of colcemid, accumulated by cells during a long-term incubation appeared to be equal; however, the initial uptake rates being higher for colcemid. The rates of the efflux of 3H-colchicine and 3H-colcemid were also different.