Destabilase-lysozyme-2 - original recombinant thrombolytic preparation of medicinal leech inhibits horse platelets aggregation.

Unlabelled: The purpose. Identifying the capacity of the medicinal leech novel original recombinant thrombolytic preparation Destabilase-Lysozyme-2 to inhibit the blood platelet aggregation.

Methods: Gene of destabilase-lysozyme.

Detection of S-adenosylhomocysteine and methylation index in blood by capillary electrophoresis.

This work proposes an approach to the direct analysis of S-adenosylhomocysteine (SAH) and the methylation index in blood using CE with UV detection (CE-UV). After application of meglumine postinjection, we achieved SAH in-capillary preconcentration in the HClO4 extracts of erythrocytes, which improved the detection limit (S/N = 3) of SAH up to 3 fmol or 180 nmol/L at the injection volume of 50 nL, taking into account the sample dilution rate. CE-UV was carried out in 30 mM glycine and 45 mmol/L HCl (pH ~1.

[Determination of reduced homocysteine fraction in blood plasma with HPLC-MS].

Homocysteine is a bioactive compound involved in many impotant processes of clinical significance. High sensitivity methods associated with preparation of oxidation-resistant derivatives are of demand for determination of different homocysteine fractions. In this work a derivatization agent N-ethylmaleinimide (NEM) was proposed by us for detection of reduced homocysteine fraction with HPLC-MS.

[Diversity and dynamics of bacteriophages in horse feces].

The complex cellulolytic microbial community of the horse intestines is a convenient model for studying the ecology of bacteriophages in natural habitats. Unlike the rumen of the ruminants, this community of the equine large intestine is not subjected to digestion. The inner conditions of the horse gut are much more stable in comparison to other mammals, due to the fact that the horse diet remains almost unchanged and the intervals between food consumption and defecation are much shorter than the whole digestive cycle.