Radiation-induced cell cycle arrests in Ehrlich ascites carcinoma cells in vivo.

Radiation-induced progression delay in G(1)/S, S and G(2)/M phases of p53 wild-type Ehrlich ascites carcinoma (EAC) cells growing in vivo was investigated by DNA flow cytometry. Different behavior patterns of EAC cells at the time after irradiation with low (2, 4, 6, 8 Gy) and high (10, 15, 20 Gy) doses were evaluated. While EAC cells showed a small progression delay in S phase and a dose-dependent block in G(2)/M phase after the irradiation with low doses, a clear additional block in G(1)/S phase was observed after irradiation with high doses.

[Main milestones of development and application of ductal cytometry at the Russian Federal Research Center for Radiology and Surgical Technology, St. Petersburg].

Ductal cytometry provides data on cellular DNA and RNA levels and overall profile of specific proteins identifiable by monoclonal antibodies. Results of its long-term use in clinical and oncological research are presented. Application of dosage ranging 0.

A number of bone marrow mesenchymal stem cells but neither phenotype nor differentiation capacities changes with age of rats.

Bone marrow (BM) derived mesenchymal stem cells (MSC) are pluripotent cells which can differentiate into osteogenic, adipogenic and other lineages. In spite of the broad interest, the information about the changes in BM cell composition, in particularly about the variation of MSC number and their properties in relation to the age of the donor is still controversial. The aim of this study was to investigate the age associated changes in variations of BM cell composition, phenotype and differentiation capacities of MSC using a rat model.

[The combained effects of the long-term gamma-irradiation and heavy metall ions on the haematopoietic system of rats].

The combiened effects of different dose rates (0.625 microGy/s - 1.1 mGy/s) of gamma-irradiation and of cuprum and of cadmium ions on the haematopoietic system of rats were studied.