Publications by authors named "A S Greenwald"
Formalin-fixed paraffin-embedded (FFPE) samples are valuable but underutilized in single-cell omics research due to their low RNA quality. In this study, leveraging a recent advance in single-cell genomic technology, we introduce snPATHO-seq, a versatile method to derive high-quality single-nucleus transcriptomic data from FFPE samples. We benchmarked the performance of the snPATHO-seq workflow against existing 10x 3' and Flex assays designed for frozen or fresh samples and highlighted the consistency in snRNA-seq data produced by all workflows.
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- Gliomas consist of malignant cells that exhibit various cellular states, which are organized in distinct ways within the tumor.
- Research reveals three key modes of organization: localized environments with predominant cellular states, specific pairings of these states residing close to one another, and a global architecture consisting of five layers driven by hypoxia.
- The study emphasizes that hypoxia influences the spatial arrangement of these cellular states, while regions far from hypoxic areas show less organization, offering a novel framework for understanding glioma structure.
Article Synopsis
- Antenatal cardiovascular disease significantly contributes to complications and deaths in pregnant women, with rheumatic mitral stenosis being particularly dangerous.
- A case of a young woman with severe pulmonary hypertension due to this condition during pregnancy illustrates the urgent need for specialized care.
- The management involved a coordinated effort from a multidisciplinary team and included advanced procedures like extracorporeal membrane oxygenation, a cesarean section, hysterectomy, and valve replacement surgery to ensure the safety of both mother and baby.
There is ample evidence to suggest that upright positions and mobility during labor improve birth outcome, including shorter duration of childbirth and reduced risk of cesarean section. The use of nonpharmacological interventions for pain management during childbirth are recommended by major health-care institutions and medical providers, however, the current methodologies for training coping strategies for use during labor have not shown to be effective on mobility or birth outcome. The purpose of this study was to apply an in-vivo teaching technology to the current childbirth model to prompt an imitative repertoire of empirically demonstrated labor coping strategies.
View Article and Find Full Text PDFObjectives: The mortuary record at Middle Period site Kalawwasa Rummeytak (CA-SCL-134) (2600-1225 cal BP) in California's southern Santa Clara Valley shows pronounced wealth inequality; Olivella shell bead wealth, as well as other grave goods, are concentrated in the burials of several older adult females. The concentration of wealth among women, along with regional strontium isotopic evidence of male-biased residential shifts in early adulthood, suggests a matrilineal kinship system that practiced matrilocal post-marital residence patterns. We suggest local resource enhancement effects incentivized keeping women in their natal communities and investing more in female offspring.
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