Alpaca single B cell interrogation and heavy-chain-only antibody discovery on an optofluidic platform.

VHH discovery approaches have been limited by the lack of methodologies for camelid B cell interrogation. Here, we report a novel application of the Beacon® optofluidic platform to the discovery of heavy-chain-only antibodies by screening alpaca B cells. Methods for alpaca B cell enrichment, culture, IgG2/3 detection, and sequencing were developed and used to discover target-specific VHH from an alpaca immunized with prostate-specific membrane antigen (PSMA) or a second target.

Erratum: Coalescence of Two Impurities in a Trapped One-Dimensional Bose Gas [Phys. Rev. Lett. 121, 080405 (2018)].

This corrects the article DOI: 10.1103/PhysRevLett.121.

Fluidic bacterial diodes rectify magnetotactic cell motility in porous environments.

Directed motility enables swimming microbes to navigate their environment for resources via chemo-, photo-, and magneto-taxis. However, directed motility competes with fluid flow in porous microbial habitats, affecting biofilm formation and disease transmission. Despite this broad importance, a microscopic understanding of how directed motility impacts the transport of microswimmers in flows through constricted pores remains unknown.

Streptococcus agalactiae clinical isolates in Northwest Iran: antibiotic susceptibility, molecular typing, and biofilm formation.

Group B Streptococcus () is one of the colonizing bacteria in pregnant women which can be a causative agent of meningitis and neonatal sepsis. This organism has also been increasingly related to invasive infections in non-pregnant adults. In present study, we aimed to characterize the clonality of biofilm-producing isolates from various sources from two different clinical laboratories in Tehran, Iran.

HIV-1 drug resistance mutations detection and HIV-1 subtype G report by using next-generation sequencing platform.

Background: Based on world health organization (WHO) recommend, drug resistance assay should be performed in initial of treatment and after treatment for administering and monitoring of anti-retroviral regime in HIV-1 infected patients.

Material And Method: NGS analyses were performed on forty-one plasma samples from HIV-1 affected patients using the Sentosa SQ HIV genotyping assay (Vela-Diagnostics, Germany). This system comprises a semi-automated Ion torrent based platform and the sequencing results were analyzed based on ANRS, REGA and Stanford drug resistance algorithms.