CHD6 has poly(ADP-ribose)- and DNA-binding domains and regulates PARP1/2-trapping inhibitor sensitivity via abasic site repair.

To tolerate oxidative stress, cells enable DNA repair responses often sensitive to poly(ADP-ribose) (PAR) polymerase 1 and 2 (PARP1/2) inhibition-an intervention effective against cancers lacking BRCA1/2. Here, we demonstrate that mutating the CHD6 chromatin remodeler sensitizes cells to PARP1/2 inhibitors in a manner distinct from BRCA1, and that CHD6 recruitment to DNA damage requires cooperation between PAR- and DNA-binding domains essential for nucleosome sliding activity. CHD6 displays direct PAR-binding, interacts with PARP-1 and other PAR-associated proteins, and combined DNA- and PAR-binding loss eliminates CHD6 relocalization to DNA damage.

BCL6 coordinates muscle mass homeostasis with nutritional states.

Nutritional status is a determining factor for growth during development and homeostatic maintenance in adulthood. In the context of muscle, growth hormone (GH) coordinates growth with nutritional status; however, the detailed mechanisms remain to be fully elucidated. Here, we show that the transcriptional repressor B cell lymphoma 6 (BCL6) maintains muscle mass by sustaining GH action.

Multiple Gas-Containing Renal Stones: A Case Report.

BACKGROUND Emphysematous urinary tract infections are rare and serious conditions that are often multifactorial in etiology and may be associated with the presence of renal stones. Diagnosis can be made by finding gas within the renal collecting system or parenchyma. However, the radiographic finding of gas within a renal stone is rare and little has been published to describe the significance of this finding, its promoting factors, and management.

Laryngeal Mucosal Tumor Mapping with Narrow Band Imaging and Confocal Laser Endomicroscopy.

Confocal laser endomicroscopy (CLE) represents an emerging probe-based intraoperative optical imaging modality, contingent on differential fluoroscein uptake, equipped to improve intraoperative identification of subclinical mucosal disease. In the analysis herein, we describe improved sensitivity, compared to narrow band imaging and small-volume tissue biopsies, of CLE in the discrimination of occult disease based on cellular and subcellular morphology during endoscopic resection of early-staged larynx cancers. Laryngoscope, 2024.

Tuning Secondary-Sphere Lewis Acidity via Late-Stage Modification of an Appended Borane.

To develop synthetic strategies to construct ligands containing secondary-sphere acids, we demonstrate that an appended borane of low Lewis acidity (-BPin) can be upgraded to a strong Lewis acid (-BF). Using a pyridine-pyrazole ligand coordinated to Mo(CO), we show that a pendent -BPin group undergoes exhaustive fluorination to -BFK, a precursor to a highly acidic -BF unit (acceptor number ∼15× greater than that of -BPin).