A spectrophotometric method for N-nicotinoyl-tryptamine (tryptamide) assay in the blood serum of rats.

A spectrophotometric method for N-nicotinoyl-tryptamine (tryptamide) (TR) assay in the blood serum of rats has been elaborated. Sensitivity of the method is approx. 1 microgram of TR/ml of blood serum, recovery approx.

Chronic dosage of imipramine in animal experiment: concentrations of imipramine and desipramine in the rat brain after various modes of dosage.

Concentrations of imipramine (IMI) and desipramine (DMI) in the rat brain after administration of IMI in aqueous solution by stomach gauge twice daily, in oil solution parenterally at 48 h intervals, and with drinking water were assayed. IMI administration with the drinking water for 21 days produced a pattern of the brain concentrations of IMI and DMI similar to that observed in rats receiving IMI by stomach tube for two weeks, at 12 h intervals. When IMI was given to rats ip in an oil solution at 48 h intervals for 10 days, the brain levels of both IMI and DMI were very high and rather stable during 48 h after administration of the last dose of IMI.

Effects of some antidepressant drugs on apomorphine concentration in the central nervous system of rats and apomorphine-induced stereotypy.

Pharmacokinetic aspects of interaction between antidepressant drugs (AD) and apomorphine (APO) have been studied. It was found that neither acute nor chronic AD pretreatment affected the distribution of APO-induced stereotypy was not changed. An exception was citalopram (CIT) which given in a single dose to rats significantly potentiated APO-induced gnawing.

Distribution of citalopram in the blood serum and in the central nervous system of rats after single and multiple dosage.

Distribution in rat tissues of citalopram, a potent and specific inhibitor of neuronal 5-HT uptake was uneven. The blood serum level of the drug did not reflect its distribution in the brain tissue and in the spinal cord. Prolonged administration of citalopram enhanced its blood serum, brain and spinal cord AUC values and slowed down its elimination from all investigated tissues.

On-line diode array UV-visible spectrometry in screening for drugs and drug metabolites by high-performance liquid chromatography.

The direct coupling of a multi-channel diode array UV-visible spectrophotometer to a powerful reversed-phase HPLC separation system is considered, especially for use in qualitative analysis, e.g., screening/identification of drugs and drug metabolites.