Measuring HSD17β13 protein turnover in mouse liver with DO metabolic labeling and hybrid LC-MS.

Background: Metabolic labeling with heavy water (DO) followed by LC-MS has become a powerful tool for studying protein turnover . Developing a quantitative method to measure partially labeled low-abundance proteins poses many challenges because heavy isotopomers of peptides, especially their changes through deuterium labeling, are difficult to detect.

Methods: A workflow that coupled immunocapture and LC-high-resolution MS to determine the synthesis rate of HSD17β13 protein in mouse liver was presented.

Antimicrobial Resistance in spp. Isolated from Native Brazilian Fish Species: A Growing Challenge for Aquaculture.

spp. has emerged as a pathogen that is affecting global aquaculture, with , , and causing piscine lactococcosis. While antimicrobials are commonly used to treat diseases in aquaculture, reports of antimicrobial resistance in fish isolates are increasing.

The Sentosa Nurses: Historical Context for Policies to Protect Internationally-Educated Nurses from Human Trafficking.

This article presents a historical analysis of the Sentosa nurses, a group of nurses recruited from the Philippines in 2005 and 2006 to work in a health-care facility on Long Island, New York. The international nurse recruitment company that hired them underpaid them, assigned them to work in unsafe conditions with low nurse-to-patient ratios, and breached other parts of their contracts with the nurses. When the nurses decided to resign and break from their contracts early, the recruitment company retaliated, initiating civil, administrative, and criminal charges against the nurses.