Technical challenges for complete implementation of automated growth-based methods for microbiological examination of advanced therapy medicinal products. What's wrong with Candida albicans?

Background: Automated growth-based methods for sterility testing of cell-therapy products should be qualified to demonstrate that they are equivalent to, or better than, the conventional reference method. The aim of the present study was to assess the ability of the BACTEC FX40 system to detect low microbial contamination and to confirm the suitability of the method in the presence of seven different human mesenchymal cell-based products, according to Ph. Eur.

A novel activity on thymocytes cells exerted by the rattlesnake (Crotalus durissus cumanensis) venom.

Introduction: The thymus is active mainly during the neonatal and pre-adolescent periods.

Objective: To test naïve thymocytes proliferation and monocytes stimulation.

Materials And Methods: We collected fresh thymus tissue from neonate mice after surgery.

Evaluation of the effectiveness of a new cryopreservation system based on a two-compartment vial for the cryopreservation of cell therapy products.

Background Aims: Successful cell cryopreservation and banking remain a major challenge for the manufacture of cell therapy products, particularly in relation to providing a hermetic, sterile cryovial that ensures optimal viability and stability post-thaw while minimizing exposure to toxic cryoprotective agents, typically dimethyl sulfoxide (MeSO).

Methods: In the present study, the authors evaluated the effectiveness and functionality of Limbo technology (Cellulis S.L.

Qualitative and Quantitative Study of the Changes in the Ultrastructure of Mammalian Adrenal Cortex Caused by the Venezuelan Tigra Mariposa () Snake Venom.

The damage of the adrenal gland by snake venoms needs to be clarified. Lethality (LD) of () venom was established by intraperitoneally mice injections. Preparation of specimens for transmission electron microscopy samples from cortex adrenal gland biopsies at 3, 6, and 24 h was processed.

Biological activities of a new crotamine-like peptide from Crotalus oreganus helleri on C2C12 and CHO cell lines, and ultrastructural changes on motor endplate and striated muscle.

Crotamine and crotamine-like peptides are non-enzymatic polypeptides, belonging to the family of myotoxins, which are found in high concentration in the venom of the Crotalus genus. Helleramine was isolated and purified from the venom of the Southern Pacific rattlesnake, Crotalus oreganus helleri. This peptide had a similar, but unique, identity to crotamine and crotamine-like proteins isolated from other rattlesnakes species.