Implementing Evidence-Based, Electronic, Substance-Use Screening in a Primary Care Clinic.

Purpose: Substance use is common among adolescents, and among those with substance use disorders; use often persists into middle age, underlying the importance of early detection and treatment. The goal of this quality improvement project was to increase the electronic substance use screening rate of adolescents presenting for preventative health visits from 0% to 50% between March 2021 and September 2021.

Methods: This is an ongoing quality improvement project.

Ubiquitous Knockdown of using TRiP.GL01126 Results in Spermatid Mislocalization During Drosophila Spermatogenesis.

METTL3 the enzyme that catalyzes the mA RNA modification in Drosophila is highly conserved and essential in various eukaryotic organisms. and its homologs have been linked to biological processes such as gametogenesis. We focused on characterizing the role of METTL3 in Drosophila spermatogenesis.

Dm Ime4 depletion affects permeability barrier and Chic function in Drosophila spermatogenesis.

Adenosine methylation of messenger RNA at the N position (mA) is a non-editing modification that can affect several aspects of mRNA metabolism. Dm Ime4, also known as METTL3, MTA, and MTA-70 in other organisms, is the catalytic subunit of the methyltransferase complex that adds this modification. Dm ime4 is evolutionarily conserved and essential for development in metazoans and plants.

The mA Dynamics of in Neurogenesis.

Our understanding of the biological role of -methyladenosine (mA), a ubiquitous non-editing RNA modification, has increased greatly since 2011. More recently, work from several labs revealed that mA methylation regulates several aspects of mRNA metabolism. The "writer" protein METTL3, known as MT-A70 in humans, Ime4 in flies, and MTA in plants, has the catalytic site of the METTL3/14/16 subunit of the methyltransferase complex that includes many other proteins.

A Direct and Simple Method to Assess Drosophila melanogaster's Viability from Embryo to Adult.

In Drosophila melanogaster, viability assays are used to determine the fitness of certain genetic backgrounds. Allelic variations can result in partial or complete loss of viability at different stages of development. Our lab has developed a method to assess viability in Drosophila from embryo to fully mature adult.