Publications by authors named "A Riabko"
Article Synopsis
- Botulinum neurotoxins (BoNTs) are highly potent natural toxins, and neutralizing monoclonal antibodies (MAbs) offer an effective immunotherapy against them, with hybridoma technology being the go-to method for MAb production.
- In this study, researchers isolated plasmablasts and activated memory B-cells from a donor who had received BoNT/A injections, then fused them with a cell line to create hybridomas that produce native human MAbs specific to BoNT/A.
- Three hybridomas were successfully created, one of which (1B9) not only binds effectively to BoNT/A but also inhibits its catalytic activity, showing promise for effective treatment even in cases of low
Production and Characterization of Rat Monoclonal Antibodies against the PAL Antigen of spp.
Mol Gen Microbiol Virol
October 2022
The purpose of this work was to obtain genus-specific monoclonal antibodies against the spp. recombinant PAL protein, which will subsequently allow to use them as a basis for the development of new express tests for pathogenic legionella detection. A short three-week immunization protocol for Wistar rats was used to generate rat-mouse heterohybridomas producing antibodies against PAL.
View Article and Find Full Text PDFNeutralization of the lethal toxin of Bacillus anthracis is an important topic of both fundamental medicine and practical health care, regarding the fight against highly dangerous infections. We have generated a neutralizing monoclonal antibody 1E10 against the lethal toxin of Bacillus anthracis and described the stages of receptor interaction between the protective antigen (PA) and the surface of eukaryotic cells, the formation of PA oligomers, assembly of the lethal toxin (LT), and its translocation by endocytosis into the eukaryotic cell, followed by the formation of a true pore and the release of LT into the cell cytosol. The antibody was shown to act selectively at the stage of interaction between Bacillus anthracis and the eukaryotic cell, and the mechanism of toxin-neutralizing activity of the 1E10 antibody was revealed.
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- Live anthrax vaccine using attenuated Bacillus anthracis spores is utilized in Russia and CIS countries to prevent anthrax.
- The study examined how long specific antibodies related to spore antigens and protective factors persist in donors after vaccination, and how these levels relate to the ability to neutralize toxins.
- Findings revealed that antibodies can last over a year post-vaccination, with a moderate correlation between antibody levels and neutralization activity in the donors’ blood.
The evolution of the beam distribution in a double-rf system with a phase modulation on either the primary or secondary rf cavity was measured. We find that the particle diffusion process obeys the Einstein relation if the phase space becomes globally chaotic. When dominant parametric resonances still exist in the phase space, particles stream along the separatrices of the dominant resonance, and the beam width exhibits characteristic oscillatory structure.
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