Publications by A Ranella

Publications by authors named "A Ranella"

Adv Healthc Mater

October 2024

Biomaterial scaffolds, like multichannel hydrogels, are being explored for their ability to help regenerate neural tissue and guide nerve growth after disruptions.
The study focuses on human amniotic membranes modified with methacryloyl domains (AMMA), which create soft, multichannel hydrogels that mimic the natural architecture of nerve tracts.
Preliminary results indicate that AMMA hydrogels support neural stem cell adhesion, growth, and the formation of functional synaptic connections, highlighting their potential for advancing regenerative neuroscience.

Bioengineering (Basel)

July 2023

The PNS can regenerate its axons after injury, but this process often fails due to misleading signals that misguide their growth.
Successful neurite guidance is crucial for neurogenesis, and microfluidic systems simulate natural body environments, optimizing nutrient delivery and waste removal.
This review explores how the physical characteristics of surfaces (topography) and fluid movement work together to influence neuronal behavior, highlighting the potential of microfluidic systems to enhance neuronal growth.

Int J Mol Sci

May 2023

This study investigated the unique morphology of 50B11 nociceptive sensory neurons in vitro, specifically their differentiation while cultured upside-down on cover glass supports.
Using advanced microscopy techniques like multi-photon non-linear and second harmonic generation (SHG) microscopy, researchers observed enhanced neurite growth and microtubule organization in these inverted neurons.
The findings suggest that inverted culture conditions could improve the 50B11 model for studying sensory neuron physiology related to various peripheral nervous system diseases and for testing analgesic drugs.

Biomater Adv

May 2023

Graphene-based materials, particularly reduced graphene oxide (rGO), present exciting opportunities for creating scaffolds in neural tissue engineering, especially when combined with decellularized extracellular matrix from adipose tissue (adECM).
The study explores how varying concentrations of rGO in scaffolds affects the structural interactions and properties, impacting cell adhesion and growth.
Higher concentrations of rGO not only promote the differentiation of neural precursor cells into neurons but also influence the behavior of astrocytes, enhancing their reactivity without triggering scar formation.

Tissue Eng Regen Med

February 2023

- The study aims to create effective scaffolds for neural tissue engineering by examining how neuronal cells react to specially patterned silicon surfaces and biodegradable materials.
- Researchers experimented with neuro2a cells alone and alongside glial cells to understand the impacts of surface roughness on cell differentiation, noting that increased roughness hinders this process.
- Findings emphasize the critical role of surface texture in neuronal cell growth and suggest further investigation could identify reasons for limited differentiation, helping to design better culture substrates.