In Vitro Mineralisation of Tissue-Engineered Cartilage Reduces Endothelial Cell Migration, Proliferation and Tube Formation.

Tissue engineering bone via endochondral ossification requires the generation of a cartilage template which undergoes vascularisation and remodelling. While this is a promising route for bone repair, achieving effective cartilage vascularisation remains a challenge. Here, we investigated how mineralisation of tissue-engineered cartilage affects its pro-angiogenic potential.

The NFIB-ERO1A axis promotes breast cancer metastatic colonization of disseminated tumour cells.

Metastasis is the main cause of deaths related to solid cancers. Active transcriptional programmes are known to regulate the metastatic cascade but the molecular determinants of metastatic colonization remain elusive. Using an inducible piggyBac (PB) transposon mutagenesis screen, we have shown that overexpression of the transcription factor nuclear factor IB (NFIB) alone is sufficient to enhance primary mammary tumour growth and lung metastatic colonization.

A dose-dependent relationship between copper burden in female urchin gonads and developmental impairment of their offspring.

We examined transgenerational effects of exposure to copper on early development of offspring in the urchin Evechinus chloroticus, and whether parental exposure mediated offspring response to a further low level exposure to copper during early development. Month-long exposure to elevated copper (25 μgL) in the laboratory increased copper in gonads of both males and females and reduced gonad size by almost half. There was a negative relationship between adult size and copper burden.