Dvl2 silencing in postdevelopmental cells results in aberrant cell membrane activity and actin disorganization.

The upstream events by which endothelial cells perceive the necessity for migration and how this signal results in coordinated movement is unknown. The synchrony underlying these events shares parallels to events occurring during the movement of tissues in embryogenesis. While Wnt signaling is an important pathway in development, components of the cascade exist in postdevelopment endothelial cells.

Subcellular distribution of Wnt-1 at adherens junctions and actin-rich densities in endothelial cells.

The Wnt family of signaling proteins functions in embryonic development and mammalian oncogenesis. It is unknown whether these molecules have a role in normal, postdevelopmental, homeostatic processes. Possessing a putative signal sequence and potential glycosylation sites, Wnt-1 is believed to be secreted and remain associated with the cell surface and extracellular matrix.

Genetic tracing of arterial graft flow surface endothelialization in allogeneic marrow transplanted dogs.

In order to trace genetically the source of fallout endothelialization on arterial grafts, six beagle dogs with successful autologous bone marrow transplantation received composite tandem aortic grafts with an isolated, totally impervious Dacron graft and a porous Dacron graft for 12 weeks. For impervious segments, five of 12 fresh tissue samples were Factor VIII/von Willebrand factor + (FVIII/vWF) and seven had faint or negative signals; three of the FVIII/vWF + samples had alpha-actin + smooth muscle cells. Polymerase chain reaction (PCR) study showed eight had a pure donor DNA genotype and four had donor/host mixed, with the donor predominant.

The effect of flow shear stress on endothelialization of impervious Dacron grafts from circulating cells in the arterial and venous systems of the same dog.

The purpose of this report was to study effects of shear force and hemodynamic conditions that influence fallout healing in the arterial and venous systems of the same dog. Knitted Dacron grafts made impervious by a 1.5 mm thick coat of silicone rubber bonded to the external surface were implanted for 4 weeks during the same surgery in the descending thoracic aorta (DTA), abdominal aorta (AA) and inferior vena cava (IVC) of each of five dogs.

Early flow surface endothelialization before microvessel ingrowth in accelerated graft healing, with BrdU identification of cellular proliferation.

The purpose of this report was to determine if flow surface endothelialization could precede microvessel ingrowth from the perigraft area in porous Dacron grafts, by using an accelerated graft healing model with short implant periods. Dacron grafts were implanted in the abdominal aorta of 22 dogs and wrapped in autogenous inferior vena cava (IVC), which provided excellent conditions for extramural angiogenesis, microvessel development, and ingrowth toward the graft. Retrieval times were 7 days (n = 4), 8 days (n = 5), 9 days (n = 4), 10 days (n = 3), 11 days (n = 4) and 12 days (n = 3) postoperatively.