Systemic candidiasis is a severe opportunistic fungal infection primarily caused by specific yeast species, with increasing cases from non- species that may resist antifungal treatments.
The study aimed to develop a PCR assay for quick detection of common pathogenic yeast species, focusing on blood and bronchial samples, using specially designed oligonucleotides that target a specific rDNA region.
The newly developed PCR method demonstrated a sensitivity of 73.9%, a specificity of 96.3%, and strong predictive values, indicating its potential for reliable use in diagnosing systemic candidiasis.