Publications by authors named "A R Puppala"
Article Synopsis
- The incorporation of selenocysteine (Sec) into the genetic code has led to the creation of a specialized group of proteins, known as the selenoproteome, across all life forms.* -
- In humans, the enzyme O-phosphoseryl-tRNASec selenium transferase (SepSecS) has a unique structure that limits its ability to bind more than two tRNASec molecules, due to a specific acidic α-helical extension.* -
- Research finds that the tRNA-binding mechanisms of SepSecS vary across species, with significant differences between mammals and archaea, indicating that the ability to regulate selenoprotein synthesis has evolved differently in these groups.*
Unlabelled: Although genomes encode instructions for mammalian cell differentiation with rich syntactic relationships, existing methods for genetically programming cells have modest capabilities for stepwise regulation of genes. Here, we developed a sequential genetic system that enables transcriptional activation of endogenous genes in a preprogrammed, stepwise manner. The system relies on the removal of an RNA polymerase III termination signal to induce both the transcriptional activation and the DNA endonuclease activities of a Cas9-VPR protein to effect stepwise progression through cascades of gene activation events.
View Article and Find Full Text PDFThe membrane interface probe (MIP) is an efficient and economical in-situ tool for chlorinated hydrocarbon (CH) contaminated site investigation. Given that the interpretation of MIP test is currently limited to a qualitative level, a theoretical model considering multiphase flow and multifield coupling is firstly proposed to simulate MIP test process. This model can consider phase change, membrane effect, adsorption and dissolution of the CH liquid, gas diffusion, and evaporation.
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- O-Phosphoseryl-tRNASec selenium transferase (SepSecS) plays a crucial role in the final step of selenocysteine synthesis in archaea and eukaryotes, highlighting its importance in maintaining the selenoproteome.
- Recent high-resolution X-ray crystallography studies reveal that the binding of tRNASec induces conformational changes in SepSecS, essential for its catalytic function, by organizing active sites and stabilizing the protein's structure.
- The formation of the productive SepSecS•tRNASec complex is driven by electrostatic interactions, providing new insights into the mechanisms behind selenocysteine formation in these organisms.
Background: Intrinsic rhythms in host and cancer cells play an imperative role in tumorigenesis and anticancer therapy. Circadian medicine in cancer is principally reliant on the control of growth and development of cancer cells or tissues by targeting the molecular clock and implementing time-of-day-based anticancer treatments for therapeutic improvements. In recent years, based on extensive high-throughput studies, we witnessed the arrival of several drugs and drug-like compounds that can modulate circadian timekeeping for therapeutic gain in cancer management.
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