Temperature-Dependent Effects on Cyclic Fatigue Resistance in Three Reciprocating Endodontic Systems: An In Vitro Study.

This study aimed to analyze the effect of 1% sodium hypochlorite (NaOCl) solution at different temperatures on endodontic file resistance to cyclic fatigue. A total of 90 files, Reciproc NiTi M-Wire (REC) (n = 30), WaveOne Gold (WOG) (n = 30), and Reciproc Blue (RB) (n = 30) were activated under constant irrigation with 1% NaOCl at 5, 37, and 60 °C in a stainless-steel artificial canal (curvature angle = 60°; radius = 5 mm). The time to the fracture and the maximum and minimum load were recorded for each instrument, and data were subjected to statistical analysis.

Longitudinal multi-omics in alpha-synuclein Drosophila model discriminates disease- from age-associated pathologies in Parkinson's disease.

Parkinson's disease (PD) starts decades before symptoms appear, usually in the later decades of life, when age-related changes are occurring. To identify molecular changes early in the disease course and distinguish PD pathologies from aging, we generated Drosophila expressing alpha-synuclein (αSyn) in neurons and performed longitudinal bulk transcriptomics and proteomics on brains at six time points across the lifespan and compared the data to healthy control flies as well as human post-mortem brain datasets. We found that translational and energy metabolism pathways were downregulated in αSyn flies at the earliest timepoints; comparison with the aged control flies suggests that elevated αSyn accelerates changes associated with normal aging.

Clinical Outcomes of a Refractive Extended Depth of Focus Intraocular Lens.

Purpose: To evaluate the clinical and patient-reported outcomes of the toric version of a refractive extended depth of focus intraocular lens (IOL).

Methods: This was a prospective observational study including 26 eyes of 26 patients (age range: 58 to 88 years) who underwent cataract surgery with implantation of the LuxSmart toric IOL (Bausch & Lomb GmbH). Changes in distance, intermediate (80 cm), and near (40 cm) visual acuities, manifest refraction, defocus curve, distance photopic contrast sensitivity (CSV-1000 test), and patient-reported outcomes (Catquest-9SF questionnaire) were evaluated during a 3-month follow-up.

Increased cancer risk in a cohort of patients with acromegaly in Israel.

The association between acromegaly and cancer had been assessed mainly in population studies with inconsistent results. The objective of this study was to investigate the risk of cancer in a large cohort of patients with acromegaly compared with matched controls. The comprehensive computerized database of the largest healthcare provider organization in Israel was searched for patients diagnosed with acromegaly in 2000-2021.

Two rare mutations in homozygosity synergize to silence TREX1 in Aicardi-Goutières syndrome.

Background: Aicardi-Goutières syndrome (AGS) is a rare monogenic type I interferonopathy characterized by dysregulated inflammation and tissue damage that primarily affects the central nervous system. AGS is genetically diverse, with pathogenic variants across multiple genes, including TREX1, which drives excessive type I interferon (IFN) production.

Objective: This study investigated the genetic and molecular mechanisms underlying AGS in a family of two affected children, focusing on the role of variants in protein expression and dysregulation of the interferon pathway.