Publications by authors named "A R Lehrer"
Multiplex immunoassays allow for the simultaneous detection of antibody populations targeting multiple antigens in a very sensitive, high-throughput format. This assay has applications in seroprevalence, serodiagnosis, and vaccine assessment with greater sensitivity and dynamic range than standard ELISAs (enzyme-linked immunosorbent assays). Here, we show in detail the methods for multiplex immunoassays to detect IgG to the Marburg virus glycoprotein.
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- * Researchers developed two types of serological assays in Liberia to assess COVID-19 exposure in four counties, analyzing 189 samples collected in summer 2021.
- * The assays revealed significant exposure to SARS-CoV-2 and other viruses, while also identifying inhibitory antibodies in the samples, which could help improve serological testing capabilities in underrepresented regions.
Quantitative immunoassays, such as the traditional enzyme-linked immunosorbent assay (ELISA), are used to determine concentrations of an antigen in a matrix of unknown antigen concentration. Magnetic immunoassays, such as the Luminex xMAP technology, allow for the simultaneous detection of multiple analytes and offer heightened sensitivity, specificity, low sample volume requirements, and high-throughput capabilities. Here, we describe a quantitative immunoassay using the Luminex MAGPIX® System to determine the antigen concentration from liquid samples with unknown concentrations.
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- - Zika virus (ZIKV) is a serious global health threat due to its association with neurological diseases like Guillain-Barré syndrome and microcephaly, and it spreads primarily through mosquito bites.
- - Accurate detection of ZIKV is complicated by its genetic similarity to other flaviviruses and the cross-reactivity of existing monoclonal antibodies (mAbs), particularly those targeting the envelope protein.
- - Researchers have developed two specific mAbs, A11 and A42, which target distinct regions of the ZIKV E protein, enhancing the tools available for research on ZIKV detection and understanding its behavior in infected cells.
Although two vaccines for Zaire ebolavirus (EBOV) have been licensed and deployed successfully to combat recurring outbreaks of Ebolavirus Disease in West Africa, there are no vaccines for two other highly pathogenic members of the Filoviridae, Sudan ebolavirus (SUDV) and Marburg marburgvirus (MARV). The results described herein document the immunogenicity and protective efficacy in cynomolgus macaques of a single-vial, thermostabilized (lyophilized) monovalent (SUDV) and bivalent (SUDV & MARV) protein vaccines consisting of recombinant glycoproteins (GP) formulated with a clinical-grade oil-in-water nanoemulsion adjuvant (CoVaccine HT™). Lyophilized formulations of the vaccines were reconstituted with Water for Injection and used to immunize groups of cynomolgus macaques before challenge with a lethal dose of a human SUDV or MARV isolate.
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