Hippocampal DNA methylation promotes contextual fear memory persistence by facilitating systems consolidation and cortical engram stabilization.

Background: Long-term fear memory storage involves gradual reorganization of supporting brain regions over time, a process termed systems consolidation. Memories initially rely on the hippocampus but gradually shift dependence to the neocortex. Although hippocampal activity drives this transfer, the molecular basis of systems consolidation is largely unknown.

Accuracy of nursing diagnoses identified at admission and discharge of patients with decompensated heart failure.

Purpose: To determine the accuracy of nursing diagnoses at hospital admission and discharge for patients with heart failure (HF).

Methods: This comparative study examined the documentation in 155 medical records of patients with an admitting diagnosis of HF during August 2018 and July 2019. An audit tool was used to record the diagnoses made by nurses during routine care at the time of admission and discharge.

Modulating gene expression as a strategy to investigate thyroid cancer biology.

Modulating the expression of a coding or noncoding gene is a key tool in scientific research. This strategy has evolved methodologically due to advances in cloning approaches, modeling/algorithms in short hairpin RNA (shRNA) design for knockdown efficiency, and biochemical modifications in RNA synthesis, among other developments. Overall, these modifications have improved the ways to either reduce or induce the expression of a given gene with efficiency and facility for implementation in the lab.

Increased NOX-dependent ROS production and proportionally enhanced antioxidant response in white adipose tissue of male rats.

Objective: This study aimed to investigate the redox balance in subcutaneous and retroperitoneal fat pads of male and female Wistar rats.

Materials And Methods: The study analyzed the activity and gene expression of the antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase, along with the production of NADPH oxidases dependent on HO and gene expression of NOX1, NOX2, and NOX4.

Results: The retroperitoneal fat pad in males compared with females had greater NOX2 and NOX4 expression, along with higher superoxide dismutase activity.