A multiparametric serum marker panel as a complementary test to mammography for the diagnosis of node-negative early-stage breast cancer and DCIS in young women.

Background: The sensitivity of mammography for the detection of small lesions, including node-negative early-stage (T1N0) primary breast cancer (PBC) and ductal carcinoma in situ (DCIS), is significantly decreased in young patients. From a clinical standpoint, an inconclusive mammogram reflects the inability of clinicians to confidently decide whether patients should be referred for biopsy or for follow-up with repeat imaging.

Methods: Specific ELISAs were developed for a panel of 13 well-recognized breast autoantigens (HSP60, FKBP52, PRDX2, PPIA, MUC1, GAL3, PAK2, P53, CCNB1, PHB2, RACK1, RUVBL1, and HER2).

A simplified CT-based definition of the supraclavicular and infraclavicular nodal volumes in breast cancer.

Purpose: The available contouring guidelines for the supraclavicular and infraclavicular lymph nodes appeared to be inadequate for their delineation on non-enhanced computed tomography (CT) scans. For this purpose, we developed delineation guidelines for the clinical target volumes (CTV) of these lymph nodes on non-enhanced CT-slices performed in the treatment position of breast cancer.

Materials And Methods: A fresh female cadaver study as well as delineation and an anatomical descriptions review were performed to propose a simplified definition of the supra- and infraclavicular lymph nodes using readily identifiable anatomical structures.

The contribution of proteomics to the identification of biomarkers for cutaneous malignant melanoma.

Despite significant advances in treatment of the melanoma during the past decade, the survival rate is little improved. A contributory factor to the poor outcome is the lack of appropriate sensitive and specific diagnostic, prognostic, and therapeutic response biomarkers. Many serum biomarkers have been evaluated in melanoma but their poor sensitivity and specificity remain serious limitations for their routine use in the clinical setting.

Proteomic analysis of differently archived breast cancer tissues.

Currently, the most common practice of human breast tissue preservation is formalin fixation which ensures good quality for histopathological analyses but damages DNA, RNA, and proteins, impairing their usefulness for molecular analysis and biomarker investigations. We investigated the potential value of a non-toxic fixative for sparing proteins preserved in paraffin-embedded breast biopsies. Specimens were fixed in formalin-free fixative prior to paraffin embedding, and then processed for quality and quantity of protein conservation.

70 Gy versus 80 Gy in localized prostate cancer: 5-year results of GETUG 06 randomized trial.

Purpose: To perform a randomized trial comparing 70 and 80 Gy radiotherapy for prostate cancer.

Patients And Methods: A total of 306 patients with localized prostate cancer were randomized. No androgen deprivation was allowed.