[Lethal intoxication with baclofen].

The objective of the present study was to select and develop simpler methods for the quantitative determination of baclofen in blood with the use of HPLC and tandem MS (MS-MS) techniques and its qualitative determination in cadaveric organs by the GC/MS technique. These mathods were shown to be suitable for the purpose of forensic medical analysis, clinical, toxicological, and therapeutic monitoring. The special emphasis is laid on the methods used to investigate the biological materials obtained from the subjects who died from baclofen intoxication.

[New method of simultaneous disobliteration of the visceral arteries and abdominal aorta].

Simultaneous correction of abnormal blood flow is necessary in the presence of combined lesions of various branches of the abdominal aorta. The problem can be solved comparatively easily by using transaortic endoarteriectomy, a technique proposed here, which consists essentially of longitudinal incision of the aortic lumen and simultaneous endoarteriectomy under the visual control of the abdominal aorta, the visceral branches and the renal arteries. Personal experience is based on 21 operations for cases of atherosclerosis and aspecific sclerotic aorta-arteritis.

[Current status of surgery of nephro-vascular hypertension].

With current angiology, the chances of identifying and treating operatively and radically patients with nephrovascular hypertension are greatly increased. Extending indications and improving technique it has been possible to operate 84% of patients with verified diagnosis of stenosis of the renal artery, according to angiographic data. Positive results have been achieved in 78% of patients.

[Effects of reduced diet and physical stress on liver lysosomes. Morphological and biochemical studies].

Groups of 15 rats each (I--non-reduced diet; II--non-reduced diet and physical stress; III--diet reduced by 40%; IV--reduced diet and physical stress) were examined after 20 and 140 days, respectively. The electron microscopic examination revealed that the most important changes had occurred in group III; there exists an excess of autophagolysosomes. The biochemical examinations show that the reduced diet (group III) also produces a considerable increase in activity of most of the lysosome enzymes in the liver.

[The biological value of fats].

Integral characteristics of animal development (weight increase, maturing of biological systems) used to date are not adequate to the requirements of detailed biological evaluation of dietary fats. The linoleic acid content in the dietary fat and the eicosatrienoic acid - eicosatetraenoic acid ratio (20:3/20:4 = HOLMAN coefficient) in the organism alone are also inadequate criteria. For this reason, the author attempted to establish metabolism-relevant law-like relationships between the fatty acid pattern of dietary fats and tissue lipids.