Zebrafish: A Cost-Effective Model for Enhanced Forensic Toxicology Capabilities in Low- and Middle-Income Countries.

Low- and middle-income countries (LMICs) are increasingly challenged by the rising burden of medicolegal cases. Traditional forensic infrastructure and in vivo rodent models often have significant limitations due to high costs and ethical concerns. As a result, zebrafish () are gaining popularity as an attractive alternative model for LMICs because of their cost-effectiveness and practical advantages.

In situ quantification of ribosome number by electron tomography.

Ribosomes, discovered in 1955 by George Palade, were initially described as small cytoplasmic particles preferentially associated with the endoplasmic reticulum (ER). Over the years, extensive research has focused on both the structure and function of ribosomes. However, a fundamental question - how many ribosomes are present within whole cells - has remained largely unaddressed.

Phase Characterization and Bioactivity Evaluation of Nucleic Acid-Encapsulated Biomimetically Mineralized ZIF-8.

Metal-organic frameworks (MOFs) provide diverse applications across a wide range of scientific disciplines, including drug/nucleic acid (NA) delivery. In the subclass of MOFs, zeolitic imidazolate framework-8 (ZIF-8) is well regarded due to its exceptional physicochemical properties. Biomolecules can be encapsulated and released under precise conditions within ZIF, making it an important material for materials science and biomedical applications.

Dynamic interplay of autophagy and membrane repair during Mycobacterium tuberculosis Infection.

Autophagy plays a crucial role in the host response to Mycobacterium tuberculosis (Mtb) infection, yet the dynamics and regulation of autophagy induction on Mtb-containing vacuoles (MCVs) remain only partially understood. We employed time-lapse confocal microscopy to investigate the recruitment of LC3B (LC3), a key autophagy marker, to MCVs at the single cell level with our newly developed workflow for single cell and single MCV tracking and fluorescence quantification. We show that approximately 70% of MCVs exhibited LC3 recruitment but that was lost in about 40% of those MCVs.