Asian elephant () seminal plasma: establishing the proteome and effect on spermatozoa when added to cryomedium.

Context The removal or supplementation of ejaculates with seminal plasma (SP) can affect cryotolerance and post-thaw survival of spermatozoa in many species. In the Asian elephant (Elephas maximus ), elucidation of the SP proteome and investigation of how it affects spermatozoa may enable improvement of cryopreservation protocols. Aims Herein, we characterise the Asian elephant SP proteome and investigate the impacts of SP on sperm cryotolerance in the presence of conspecific or heterospecific SP.

Non-targeted proteomic analysis of Asian elephant () seminal plasma using an in-solution digestion technique and liquid chromatography tandem-mass spectrometry.

Seminal plasma proteins have recently been reported to play a significant role as valuable materials for understanding male reproductive biology, identifying causes of fertility problems, and developing reproductive biomarkers. Proteomic analysis of seminal plasma holds promise in advancing the understanding of male Asian elephant reproductive biology. This study aims to explore seminal plasma proteins of Asian elephants and their probable functions to provide fundamental information about male reproduction in this species.

Ovarian activity in crossbred Thai native does during naturally occurring foot-and-mouth disease (FMD) virus infection.

This study aimed to compare the follicular dynamics in goats during naturally infected with foot-and-mouth disease virus (FMD) subjected to induced ovulations, and after disease recovery, crossbred Thai native does were synchronized with CIDR for 14 days, and then PGF and PMSG were administered on the day following CIDR removal. The ovarian activity was determined by transrectal ultrasonography. Clinical signs (fever, anorexia, lameness, and foot lesion) were observed on day 12 post-estrus (day 0, day of expected estrus).

Supplemented stallion seminal plasma can improve impaired motility due to the dilution effect in chilled Asian elephant sperm.

The dilution effect and effect of restoring seminal plasma (SP) proportion in diluted semen were determined in chilled Asian elephant sperm. Semen was collected from eight males, and samples with ≥30% motile sperm were used in the study. Tris-glucose-egg yolk extender (TE) was used for cooled storage at 4°C for 48 hr.

The presence of seminal plasma, especially derived from stallion semen, helps preserve chilled Asian elephant (Elephas maximus) sperm motility.

The effects of seminal plasma (SP), derived from autologous, homologous and heterologous species (stallion, boar and dog) on chilled Asian elephant sperm quality, were determined. Semen was collected from eight males and samples with ≥30% motile spermatozoa were used in the study. Semen was diluted with Tris-glucose-egg yolk extender, supplemented with different SP types and preserved at 4°C for 48 hr.