This article presents a novel and selective electrochemical bioassay with antibody and laccase for the determination of free thyroid hormone (free triiodothyronine, fT3). The biosensor was based on a glassy carbon electrode modified with a FeO@graphene nanocomposite with semiconducting properties, an antibody (anti-PDIA3) with high affinity for fT3, and laccase, which was responsible for catalyzing the redox reaction of fT3. The electrode modification procedure was investigated using a cyclic voltammetry technique, based on the response of the peak current after modifications.
View Article and Find Full Text PDFIn our previous paper we have modelled a dielectrophoretic force (DEP) and cell particle behavior in a microfluidic channel (Weber MU2023 Chip for dielectrophoretic microbial capture, separation and detection I: theoretical basis of electrode designthis issue). Here we test and confirm the results of our modeling work by experimentally validating the theoretical design constraints of the ring electrode architecture. We have compared and tested the geometry and particle capture and separation performance of the two separate electrode designs (the ring and dot electrode structures) by investigating bacterial motion in response to the applied electric field.
View Article and Find Full Text PDFWe model the dielectrophoretic response ofbacterial cells and red blood cells, upon exposure to an electric field. We model the separation, capture, and release mechanisms under flow conditions in a microfluidic channel and show under which conditions efficient separation of different cell types occurs. The modelling work is aimed to guide the separation electrode architecture and design for experimental validation of the model.
View Article and Find Full Text PDFBacterial culture methods, e.g. Plate Counting Method (PCM), are a gold standard in the assessment of microbial contamination in multitude of human industries.
View Article and Find Full Text PDFCorn processing generates thousands of tons of cob husks, which still contains many valuable elements. To make the most of these wastes, they are applied as substrates for biotransformation's procedures. This approach allowed converting or releasing, the elements deposited in the plant material and obtaining valuable products.
View Article and Find Full Text PDF